mirror of
https://github.com/NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273.git
synced 2024-06-29 07:29:01 +00:00
355f94eafc
* Cleaned up and fixed the raw text files (some features such as the start codon were misplaced) * Fixed moderna.gb (it had the BioNTech/Pfizer sequence) * Added BioNTech/Pfizer Genbank file and FASTA files for both vaccines
117 lines
7.8 KiB
Plaintext
117 lines
7.8 KiB
Plaintext
LOCUS 4004 bp RNA linear SYN 23-MAR-2021
|
|
DEFINITION Moderna mRNA-1273 vaccine, spike-encoding contig
|
|
SOURCE synthetic construct
|
|
ORGANISM synthetic construct
|
|
other sequences; artificial sequences.
|
|
REFERENCE 1 (bases 1 to 8179)
|
|
AUTHORS Jeong DE, McCoy M, Artiles K, Ilbay O, Fire A, Nadeau K, Park H,
|
|
Betts B, Boyd S, Hoh R, and Shoura M
|
|
TITLE Assemblies of putative SARS-CoV2-spike-encoding mRNA sequences for
|
|
vaccines BNT-162b2 and mRNA-1273. (version 0.1Beta 03/23/21)
|
|
FEATURES Location/Qualifiers
|
|
source 1..4004
|
|
/organism="synthetic construct"
|
|
/mol_type="other RNA"
|
|
gene 1..4004
|
|
/gene="spike glycoprotein"
|
|
/db_xref="GeneID:43740568"
|
|
5'UTR 1..57
|
|
CDS 58..3879
|
|
/codon_start=1
|
|
/product="spike glycoprotein"
|
|
/translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFR
|
|
SSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIR
|
|
GWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
|
|
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQ
|
|
GFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFL
|
|
LKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITN
|
|
LCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCF
|
|
TNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYN
|
|
YLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPY
|
|
RVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFG
|
|
RDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAI
|
|
HADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR
|
|
RARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTM
|
|
YICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFG
|
|
GFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFN
|
|
GLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQN
|
|
VLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGA
|
|
ISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMS
|
|
ECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAH
|
|
FPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELD
|
|
SFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELG
|
|
KYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSE
|
|
PVLKGVKLHYT"
|
|
3'UTR 3880..3995
|
|
polyA_signal 3996..4004
|
|
|
|
ORIGIN
|
|
1 gggaaataag agagaaaaga agagtaagaa gaaatataag accccggcgc cgccaccatg
|
|
61 ttcgtgttcc tggtgctgct gcccctggtg agcagccagt gcgtgaacct gaccacccgg
|
|
121 acccagctgc caccagccta caccaacagc ttcacccggg gcgtctacta ccccgacaag
|
|
181 gtgttccgga gcagcgtcct gcacagcacc caggacctgt tcctgccctt cttcagcaac
|
|
241 gtgacctggt tccacgccat ccacgtgagc ggcaccaacg gcaccaagcg gttcgacaac
|
|
301 cccgtgctgc ccttcaacga cggcgtgtac ttcgccagca ccgagaagag caacatcatc
|
|
361 cggggctgga tcttcggcac caccctggac agcaagaccc agagcctgct gatcgtgaat
|
|
421 aacgccacca acgtggtgat caaggtgtgc gagttccagt tctgcaacga ccccttcctg
|
|
481 ggcgtgtact accacaagaa caacaagagc tggatggaga gcgagttccg ggtgtacagc
|
|
541 agcgccaaca actgcacctt cgagtacgtg agccagccct tcctgatgga cctggagggc
|
|
601 aagcagggca acttcaagaa cctgcgggag ttcgtgttca agaacatcga cggctacttc
|
|
661 aagatctaca gcaagcacac cccaatcaac ctggtgcggg atctgcccca gggcttctca
|
|
721 gccctggagc ccctggtgga cctgcccatc ggcatcaaca tcacccggtt ccagaccctg
|
|
781 ctggccctgc accggagcta cctgacccca ggcgacagca gcagcgggtg gacagcaggc
|
|
841 gcggctgctt actacgtggg ctacctgcag ccccggacct tcctgctgaa gtacaacgag
|
|
901 aacggcacca tcaccgacgc cgtggactgc gccctggacc ctctgagcga gaccaagtgc
|
|
961 accctgaaga gcttcaccgt ggagaagggc atctaccaga ccagcaactt ccgggtgcag
|
|
1021 cccaccgaga gcatcgtgcg gttccccaac atcaccaacc tgtgcccctt cggcgaggtg
|
|
1081 ttcaacgcca cccggttcgc cagcgtgtac gcctggaacc ggaagcggat cagcaactgc
|
|
1141 gtggccgact acagcgtgct gtacaacagc gccagcttca gcaccttcaa gtgctacggc
|
|
1201 gtgagcccca ccaagctgaa cgacctgtgc ttcaccaacg tgtacgccga cagcttcgtg
|
|
1261 atccgtggcg acgaggtgcg gcagatcgca cccggccaga caggcaagat cgccgactac
|
|
1321 aactacaagc tgcccgacga cttcaccggc tgcgtgatcg cctggaacag caacaacctc
|
|
1381 gacagcaagg tgggcggcaa ctacaactac ctgtaccggc tgttccggaa gagcaacctg
|
|
1441 aagcccttcg agcgggacat cagcaccgag atctaccaag ccggctccac cccttgcaac
|
|
1501 ggcgtggagg gcttcaactg ctacttccct ctgcagagct acggcttcca gcccaccaac
|
|
1561 ggcgtgggct accagcccta ccgggtggtg gtgctgagct tcgagctgct gcacgcccca
|
|
1621 gccaccgtgt gtggccccaa gaagagcacc aacctggtga agaacaagtg cgtgaacttc
|
|
1681 aacttcaacg gccttaccgg caccggcgtg ctgaccgaga gcaacaagaa attcctgccc
|
|
1741 tttcagcagt tcggccggga catcgccgac accaccgacg ctgtgcggga tccccagacc
|
|
1801 ctggagatcc tggacatcac cccttgcagc ttcggcggcg tgagcgtgat caccccaggc
|
|
1861 accaacacca gcaaccaggt ggccgtgctg taccaggacg tgaactgcac cgaggtgccc
|
|
1921 gtggccatcc acgccgacca gctgacaccc acctggcggg tctacagcac cggcagcaac
|
|
1981 gtgttccaga cccgggccgg ttgcctgatc ggcgccgagc acgtgaacaa cagctacgag
|
|
2041 tgcgacatcc ccatcggcgc cggcatctgt gccagctacc agacccagac caattcaccc
|
|
2101 cggagggcaa ggagcgtggc cagccagagc atcatcgcct acaccatgag cctgggcgcc
|
|
2161 gagaacagcg tggcctacag caacaacagc atcgccatcc ccaccaactt caccatcagc
|
|
2221 gtgaccaccg agattctgcc cgtgagcatg accaagacca gcgtggactg caccatgtac
|
|
2281 atctgcggcg acagcaccga gtgcagcaac ctgctgctgc agtacggcag cttctgcacc
|
|
2341 cagctgaacc gggccctgac cggcatcgcc gtggagcagg acaagaacac ccaggaggtg
|
|
2401 ttcgcccagg tgaagcagat ctacaagacc cctcccatca aggacttcgg cggcttcaac
|
|
2461 ttcagccaga tcctgcccga ccccagcaag cccagcaagc ggagcttcat cgaggacctg
|
|
2521 ctgttcaaca aggtgaccct agccgacgcc ggcttcatca agcagtacgg cgactgcctc
|
|
2581 ggcgacatag ccgcccggga cctgatctgc gcccagaagt tcaacggcct gaccgtgctg
|
|
2641 cctcccctgc tgaccgacga gatgatcgcc cagtacacca gcgccctgtt agccggaacc
|
|
2701 atcaccagcg gctggacttt cggcgctgga gccgctctgc agatcccctt cgccatgcag
|
|
2761 atggcctacc ggttcaacgg catcggcgtg acccagaacg tgctgtacga gaaccagaag
|
|
2821 ctgatcgcca accagttcaa cagcgccatc ggcaagatcc aggacagcct gagcagcacc
|
|
2881 gctagcgccc tgggcaagct gcaggacgtg gtgaaccaga acgcccaggc cctgaacacc
|
|
2941 ctggtgaagc agctgagcag caacttcggc gccatcagca gcgtgctgaa cgacatcctg
|
|
3001 agccggctgg accctcccga ggccgaggtg cagatcgacc ggctgatcac tggccggctg
|
|
3061 cagagcctgc agacctacgt gacccagcag ctgatccggg ccgccgagat tcgggccagc
|
|
3121 gccaacctgg ccgccaccaa gatgagcgag tgcgtgctgg gccagagcaa gcgggtggac
|
|
3181 ttctgcggca agggctacca cctgatgagc tttccccaga gcgcacccca cggagtggtg
|
|
3241 ttcctgcacg tgacctacgt gcccgcccag gagaagaact tcaccaccgc cccagccatc
|
|
3301 tgccacgacg gcaaggccca ctttccccgg gagggcgtgt tcgtgagcaa cggcacccac
|
|
3361 tggttcgtga cccagcggaa cttctacgag ccccagatca tcaccaccga caacaccttc
|
|
3421 gtgagcggca actgcgacgt ggtgatcggc atcgtgaaca acaccgtgta cgatcccctg
|
|
3481 cagcccgagc tggacagctt caaggaggag ctggacaagt acttcaagaa tcacaccagc
|
|
3541 cccgacgtgg acctgggcga catcagcggc atcaacgcca gcgtggtgaa catccagaag
|
|
3601 gagatcgatc ggctgaacga ggtggccaag aacctgaacg agagcctgat cgacctgcag
|
|
3661 gagctgggca agtacgagca gtacatcaag tggccctggt acatctggct gggcttcatc
|
|
3721 gccggcctga tcgccatcgt gatggtgacc atcatgctgt gctgcatgac cagctgctgc
|
|
3781 agctgcctga agggctgttg cagctgcggc agctgctgca agttcgacga ggacgacagc
|
|
3841 gagcccgtgc tgaagggcgt gaagctgcac tacacctgat aataggctgg agcctcggtg
|
|
3901 gcctagcttc ttgccccttg ggcctccccc cagcccctcc tccccttcct gcacccgtac
|
|
3961 ccccgtggtc tttgaataaa gtctgagtgg gcggcaaaaa aaaa
|
|
//
|